Quick Tips to Control Flour Beetle Infestation

If detected at early stages of infestation, flour beetles can be eliminated from your home through easy, convenient methods that do not require the use of chemical pesticides.

Detection & Control

  • Inspect all stored grain products in your pantry. This includes bags of rice, flour (especially wheat), corn meal, cereals, pasta, pet foods, and anything else that is a dry grain. Take a large scoopful from each opened container, carefully sift through the content and look for any flour beetles (here’s a guide on how to identify them).
  • If flour beetles are detected in any stored grain products, immediately throw that product out and clean/vacuum the surrounding areas. Be sure to check that adjacent products are also not infested and remind yourself to not buy that specific product or avoid similar products from the same store.
  • Keep your pantry dry. Flour beetles favor moist environments and moist grain products are ideal habitats for them.
  • When buying grain products, inspect them thoroughly to detect for any holes, cracks, openings in the packaging.
  • Seal close any opened grained product either by pouring them in Ziploc bags, air-tight containers, or use a binder clip to keep the bags closed tightly.

Traps & Sprays

  • Beetle traps (such as EcoSafe “Sticky” Bug Trap or Pro Pest Pantry Moths & Beetle Trap) are made of cardboard and lined on the inside with pheromone laced sticky strips. The pheromone attracts nearby flour beetles to the trap where they get stuck on the glue strip.
  • Recent study found that pheromone based traps work better when consistent airflow is present (Campbell 2012).
  • Traps are non-toxic, easy to use, and disposable. They work great in areas where you detect there may be the presence of a flour beetle population.
  • Spray cracks and crevices with insectides (such as Demon WP, PERMA-DUST, or Ultracide) that kill any hiding adult flour beetles.
  • Insecticide can be toxic so be sure to remove all food items, utensils, dishes from the vicinity before spraying. Also be sure to only spray in cracks and not over entire surfaces.
  • Remember: insecticides should only be used as a last resort. If possible, call a professional pest control company to deal with large infestations.

References:

Campbell, J.F. 2012. Attraction of Walking Tribolium castaneum Adults to Traps. J Stored Prod Res, 51:11-22.

http://www.epestsupply.com/red-confused-flour-beetles.php#.UMkBUoPoSsf

Click to access flourbee.pdf


Coevolution with parasites drive reproductive isolation

Reproductive isolation entails a set of mechanisms, behaviors, and physiological processes that prevent members of two different species from mating and producing fertile offspring (Barton 1986). Over time, these barriers block gene flow between populations and act as reproductive boundaries that leads to the evolution of new species. Reproductive isolation can evolve in species whose geographic distribution overlaps (sympatric speciation) or when species are subdivided into geographically isolated populations (allopatric speciation).

Credit: Pearson Education, Inc.

For species with populations isolated by geographical barriers, variation in host-parasite interactions can potentially lead to rapid host population divergence. Parasites might represent at least 50% of the known biodiversity and this ubiquity makes parasites a major force in shaping genetic diversity (Schmid-Hempel 2011). The co-evolution of hosts and parasites may contribute considerably to host population diversification and eventually speciation.

Berenos et al., from the Institute of Integrative Biology – Zurich, conducted a study to test whether the biotic interactions in host-parasite co-evolution can lead to reproductive isolation via local adaptation. The experiment paired five lines of our beloved red flour beetle (Tribolium castaneum) with their respective  microsporidia, Nosema whitei. After 17 generations of species host-parasite interactions between these five lines, virgin females and males from each line were selected to mate with others from each of the four different lines and within their respective lines.

Light microscopy of N. whitei

The research team found that mating pairs from different lines had, on average, 16% fewer offspring than pairs from the same respective line. The number of offspring from the mixed mating pair was significantly lower compared to the related mating pair.

Positive correlation between coevolution and reproductive isolation.

The findings of this study suggest a role for co-evolution with parasites in in host reproductive isolation. And because reproductive isolation is a decisive step towards speciation, parasites may also accelerate population differentiation and ultimately lead to speciation.

References:

Berenos, C., Schmid-Hepel, P., Wegner, K.M. 2012. Antagonistic Coevolution Accelerates the Evolution of Reproductive Isolation in Tribolium CastaneumAm Soc Nat, 180(4): 520-528.

Barton N., Bengtsson B. O. 1986. The barrier to genetic exchange between hybridising populations. Heredity 57 (3): 357–376.

Schmid-Hempel, P. 2011. Evolutionary parasitology: the integrated study of infections, immunology, ecology and genetics. Oxford
University Press, Oxford.


Can Chrysanthemum control flour beetle infestation?

Confused flour beetles (Tribolium confusum) are one of the most serious pests in stored grain since it can easily live and feed off damaged grain products. Flour beetles are highly resistant to traditional insecticides, so farmers and scientists are constantly searching for new chemicals and methods to fend off these resilient pests. This proves to be a difficult undertaking as any novel insect control agents must be safe enough to not cause damage to stored grains and agriculture while still be potent enough to ward off the indestructible king of insects.

A joint-team from the University of Sousse and University of Pisa, led by Dr. Haouas, has recently discovered the deterrent effects of Chrysanthemum on confused flour beetles. In their study, published this year in the Journal of Pest Science, the researchers analyzed essential oils from three species of Chrysanthemum (C. coronarium, C. fuscatum, and C. grandiflorum) and for their toxicity and anti-feeding properties. Essential oils are volatile liquid compounds extracted from plants via distillation; the term is derived from the “essence” or aroma of specific plants.

C. coronarium

Image of Chamaemelum fuscatum

C. fuscatum

C. grandiflorum

The flour beetles were starved for 24 hours to induce feeding on the Chrysanthemum-laced flour. Essential oils from the leaves and flowers of each Chrysanthemum species were tested for their effects on confused flour beetle’s mortality and toxicity.

The winner of the three Chrysanthemum species proved to be C. grandiflorum. Essential oils from the leaves of this species was most effective in inhibiting the relative growth rate, efficiency of conversion of ingested food, relative consumption rate, caused an anti-feeding effect, and a high mortality (80%) of T. confusum larvae.

The runner-up was C. coronarium flowers which had essential oils that demonstrated significant lethal toxic effects to flour beetle larvae both through contact and gaseous fumigation.

C. fuscatum was also found to be particularly rich in limonene which is one of the most toxic compounds to T. confusum.

A drop a day, keeps the flour beetles away!

The insecticidal characteristics of Chrysanthemum on flour beetles should increase the use of natural plant materials in pest control as potent alternatives to synthetic pesticides. This technique offers important implications for poor farmers who cannot afford commercial pesticides to banish flour beetles through traditional methods of pest control, such as pouring a few drops of Chrysanthemum oil in stored grain.

References:

Haouaus D., Cioni P.L., Ben Halima-Kamel M., Flamini, G., Ben Hamouda M.H. 2012. Chemical composition and bioactivities of three Chrysanthemum essential oils against Tribolium confusumJ Pest Sci 85(3): 267-279.

Stamopoulos DC, Damos P, Karagianidou G. 2007. Bioactivity of five monoterpenoid vapours to Tribolium confusum (du Val) (Coleoptera: Tenebrionidae). J Stored Prod Res 43:571–577.


Tribolium 102: Sexing Flour Beetles

Now that we are familiar with identifying flour beetles out in the “field” and distinguishing them from other beetles, let’s level up our entomology stats and learn how to separate flour beetles by sex.

Adult male and female flour beetles are physically, nearly identical so the sexing of flour beetles is usually done in the pupal stage where males and females have prominent different features.

Let’s use the Confused Flour Beetle (Tribolium confusum) as example.

Flour beetles are tiny creatures and their appendages even smaller, so all sexing must be done under a light microscope. We want the beetle pupa to be on its back with the ventral side up.

Our focus is on the tip of the abdomen where there are 2 long projections called the urogomphi; both females and males have these protrusions. Layered on top the urogomphi and a little further up the abdomen is another set of 2 smaller projections called the genital papillae – this is what we will be looking at to distinguish between males and females.

On females, the genital papillae are pointy, with 2 darker dots on the tip of each, and roughly half the size of the urogomphi (they resemble tiny fingers).

On males, the genital papillae are stubbyconjoined, and barely noticeable. If female papillae resemble fingers, these look more like 2 conjoined thumbs.

Females have finger-like papillae (left), while males have stub-like projections (right). | Scientific Reports

 

 

 

 

 

 

 

 

 

References:

Park, Thomas. 1934. Observations on the General Biology of the Flour Beetle, Tribolium confusumQuarterly Review of Biology, 9(1): 36-54.

Shukla, J.N. and Palli, S. R. 2012. Sex Determination in Beetles: Production of all male progeny by Parental RNAi knockdown of transformer. Scientific Reports, 2: 602.


MythBusters Showdown: Flour beetles vs Cockroaches

The myth that cockroaches will be able to outlive all organisms on Earth seem to have originated with the development of the atom bomb. Cockroaches were reported to have survived the blasts at Hiroshima and Nagasaki which lead to the popular theory that cockroaches will come to inherit the Earth in the event that all humans are eradicated after a nuclear warfare.

Cockroaches are certainly more resistant to radiation than humans and nearly all non-insect animals. Roaches have a simple genome with fewer genes that might develop mutation due to radiation. Their cells also divide much slower than human cells (only each time it molts) which allows them more time to fix genetic defects. As a result, cockroaches can withstand radiation levels up to 15 times the lethal dose to humans.

But are cockroaches the toughest, baddest creatures out there? Or might the underrated and often overlooked flour beetles challenge them for post-Apocalyptic dominance?

The team at MythBusters aimed to test who will be the ultimate survivors in a radiation showdown between cockroaches, flour beetles, and fruit flies. So who finally took the crown for King of the (post-human) World??

You guessed it…Flour Beetles!

30 days after exposure to 10,000 rad, cockroaches had a 30% survival rate while flour beetles showed a 94% survival rate. Even more incredible, at 100,000 rad, 10% of flour beetles still lived while all the cockroaches died. That’s 100,000 times the lethal dose of radiation to humans!

Here is the video:

 

References:

Schweid, R. 1999. The Cockroach Papers: A Compendium of History and Lore. Basic Books, Inc.

Bracker, M. 1962. Atomic War Held Threat to Nature. New York Times, pp. 46.


Tribolium 101: Identifying a Flour Beetle

The Tribolium genus contains five selected species:
  • Tribolium castaneum – red flour beetle
  • Tribolium confusum – confused flour beetle
  • Tribolum destructor – destructive flour beetle
  • Tenebrio molitor – mealworm beetle
  • Tenebrio obscurus – mini mealworm

Each species possess distinctive physical (and biological) features, but all share common traits that groups them into the Tribolium genus.

Flour beetles are holometabolic meaning they undergo the complete metamorphosis life cycle from egg -> larva -> pupa -> adult.

The life span of an average adult flour beetle is approximately three years. A single adult female can lay up to 450 eggs in her lifetime at a rate of 2 to 3 eggs a day. Eggs will hatch into larvae into 5 to 12 days and become full-grown in about 30 days. The larval stage usually goes through 7 to 8 instars and grow to around 3/16 of an inch with a yellow tinge. Flour beetles are most voracious eaters during their larvae phase. Matured larva transform into cocooned pupa which is when flour beetles are most easily identified of their gender (we will see how to identify the sex of a flour beetle in a later post). From the pupa emerged the new adult beetle and the entire cycle from egg to adult takes around 6 weeks under optimal conditions.

Destructive (or dark) flour beetle pupa hatching to an adult

Let us first analyze the similarities and differences between the two most common flour beetle species: the red flour beetle and the confused flour beetle.

Red Flour Beetle (Tribolium castaneum) vs Confused Flour Beetle (Tribolium confusum)

Both species are nearly identical in physical composition. In fact, the “confused” flour beetle is named due to it being confused with the red flour beetle.

Both species are reddish-brown in color and about 1/8 of an inch in length. They leave in the same environments and compete for the same resources. The egg, larva, and pupa stages of both species are also very similar. The eggs are white, microscopic, and covered in sticky secretion with adheres flour bits. The larva phase has a creamy yellow color and two dark pointed projections at its posterior. The pupa is a lighter shade of yellow and looks similar to the Pokemon, Kakuna.

   

The defining differences between these two species is evident in their antenna. The antenna of the red flour beetle ends in a 3-segmented club and the sides of the thorax are slightly curved. The confused flour beetle has no apparent club on the 4-segmented antennae and the sides of the thorax are straighter. One additional distinction is that the red flour beetle may fly, especially before a storm, but the confused flour beetle does not fly.

Red flour beetle (left) and Confused flour beetle (right) | Purdue

Destructive flour beetle (Tribolium destructor), Mealworms (Tenebrio molitor), and Mini Mealworms (Tenebrio obscurus)

These three species are distinctive due to their dark-brown coloration.

The destructive flour beetle got its name due to the destructive damage it causes to stored grain products.

destructive or darkling flour beetle (Tribolium destructor)

Mealworms and its miniature sister species are high in protein content and makes them great food sources for reptile, fish, and bird pets.

Many cultures also consider mealworms to be delicacies and have even been incorporated into tequila-flavored novelty candies.

 

Tequila flavored mealworm (top) and mealworm-covered caramel apples (bottom)

References:

Rebecca Baldwin and Thomas R. Fasulo.  2003. “Confused Flour Beetle, Tribolium confusum and Red Flour Beetle, Tribolium castaneum”. Entomology and Nematology Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville.

Dennis Calvin. 2001. “Entomological Notes”. Department of Entomology, College of Agricultural Sciences, The Pennsylvania State University.


What are flour beetles?

My first contact with flour beetles, unknown to my non-entomology background at the time, was when I was helping my mother make rice as a kid only to find a couple tiny little dark bugs crawling around in the scoop of rice. If this experience sounds familiar then congratulations (?), you’ve came into personal contact with one of the most widely studied beetles.

red flour beetles (Tribolium castaneum) | thenakedscientists.com

There are officially 5 species of flour beetles in the Tribolium genus and they are so aptly named because they spend their entire lives in flour (and other types of grain). These creatures live, feed, reproduce, and complete their entire life cycles in their habitat. Similar to most beetles, flour beetles have a holometabolic life cycle which includes 4 life stages – egg, larva, pupa, and adult.

Life cycle of a flour beetle | University of Florida

Due to their low-maintenance lifestyles, flour beetles have become a major pest in the agriculture industry as they are easily transported around the world through grains and voraciously attacks and stored grains and foods. They generally feed on finely ground or damaged starch materials, but cannot attack whole grains. Additionally, these beetles are highly resistant to insecticides and adaptable to extreme environments. They’ve been tested to withstand radiation levels even higher than the infamously indestructible cockroaches!

The genus Tribolium has its origins in India and Africa (possibly Ethiopia). Interestingly, all members of the genus originally inhabited rotting logs and under bark of trees. Compared to day, almost all species can be found in common household grains. With the overproduction of stored grains, these invasive critters have now established their presence in all continents except Antarctica (but it doesn’t seem so daunting for these seemingly invincible bugs).  In the U.S., flour beetle distribution is primarily found in the Southern states where they favor the temperate climate, but will survive the harsh winters in the North (especially where there is central heating, which is everywhere).

Now that you’ve learned a bit on the background of these special beetles, I hope you will be more keen in keeping an eye for them next time you bake . Despite their small stature, these creatures epitomizes the phrase “big things come in small packages” as we begin to unfold their many fascinating characteristics.

So eerie…so fascinating

References:

Peter S. Dawson. 1977. Life History Strategy and Evolutionary History of Tribolium Flour Beetles. Evolution 31(1) :226-229.
Rebecca Baldwin and Thomas R. Fasulo.  2003. Confused Flour Beetle and Red Flour Beetle. Entomology and Nematology Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville.

Welcome to the World of Flour Beetles

This blog was created as my final project for the EBIO 412 course at Rice University. I will be introducing the many interesting species of the Tribolium genus, the common evolutionary, genetic, and social traits shared by these beetles, as well as novel discoveries found in ecological research of these curious creatures.

Thank you for taking the time to view my blog and hopefully you learned something new!